|
KMID : 1147720140070040202
|
|
Journal of Acupuncture and Meridian Studies
2014 Volume.7 No. 4 p.202 ~ p.210
|
|
|
In Vitro Antioxidant Activity and Effect of Parkia biglobosa Bark Extract on Mitochondrial Redox Status
|
|
Komolafe Kayode
Olaleye Tolulope Mary
Omotuyi Olaposi Idowu
Boligon Aline Augusti
Athayde Margareth Linde
Akindahunsi Akintunde Afolabi
da Rocha Joao Batista Teixeira
|
|
|
Abstract
|
|
|
|
Aqueous-methanolic extract of Parkia biglobosa bark (PBB) was screened for its polyphenolic constituents, in vitro antioxidant activity, and effect on mitochondria redox status. The in vitro antioxidant activity was assessed by using the scavenging abilities and the reducing powers of 1,1-diphenyl-2-picrylhydrazyl radical (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) diammonium salt radical cation against Fe3+. Subsequently, the ability of PBB to inhibit lipid peroxidation induced by FeSO4 (10 μm) and its metal-chelating potential were investigated. The effects of the extract on basal reactive oxygen species (ROS) generation and on the mitochondrial membrane potential (ΔΨm) in isolated mitochondria were determined by using 2′, 7′-dichlorodihydrofluorescin (DCFH) oxidation and safranin fluorescence, respectively. PBB mitigated the Fe(II)-induced lipid peroxidation in rat tissues and showed dose-dependent scavenging of DPPH (IC50: 98.33 ± 10.0 μg/mL) and ABTS. (trolox equivalent antioxidant concentration, TEAC value = 0.05), with considerable ferric-reducing and moderate metal-chelating abilities. PBB caused slight decreases in both the liver and the brain mitochondria potentials and resulted in a significant decrease (p < 0.001) in DCFH oxidation. Screening for polyphenolics using high-performance liquid chromatography coupled to a diode array detector (HPLC-DAD) revealed the presence of caffeic acid, gallic acid, catechin, epigalocatechin, rutin, and quercetin. These results demonstrate for the first time the considerable in vitro antioxidant activity and favorable effect of PBB on mitochondria redox status and provide justification for the use of the plant in ethnomedicine.
|
|
|
KEYWORD
|
|
|
antioxidant, extract, HPLC, mitochondria, Parkia biglobosa, phenolics
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|